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Product Name
Annexin V Recombinant Protein（ FITC）
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Gene ID
308
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SwissProt ID
P08758
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Gene Name
ANXA5
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Alternative Names
Annexin A5,ANXA5,Anchorin CII,Annexin-5,Calphobindin I (CPB-I),Endonexin II,ANX5, ENX2, PP4
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Background
Annexins are a family of calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS) to identify apoptotic cells. In healthy cells, PS is predominantly located along the cytosolic side of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution in the phospholipid bilayer and translocates to the extracellular membrane, which is detectable with fluorescently labeled Annexin V. In early stages of apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI) and 7-AAD, therefore cells which display only Annexin V staining (PI/7-AAD negative) are in early stages of apoptosis. During late-stage apoptosis, loss of cell membrane integrity allows Annexin V binding to cytosolic PS, as well as cell uptake of PI and 7-AAD. Annexin V staining, paired with 7-AAD or PI is widely used to identify apoptotic stages by flow cytometry.
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Research Field
Cell Biology
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Product Categories
Protein
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Host
E. coli
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Reactivity
Human, Mouse, Rat
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Application
FC
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Purity
>89% by SDS-PAGE and RP-HPLC
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Immunogen
A synthesized peptide derived from Human Annexin V
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Purification
Affinity Purified
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Conjugation
FITC
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Modification
Unmodified
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Form
Liquid
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Buffer System
TBS, pH 7.4, with 1% BSA and 0.02% sodium azide
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Storage
4° C, store in dark, DO NOT FREEZE!