产品详情
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Product Name
Immunohistochemical Chromogenic Kit (Two-Step)
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Gene ID
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SwissProt ID
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Gene Name
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Alternative Names
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Background
This product utilizes a dextran-based polymer conjugated with multiple horseradish peroxidase (HRP) molecules and antibodies. The polymer increases the enzyme density on the secondary antibody, establishing a high-sensitivity, ready-to-use, non-biotin detection system for immunohistochemical staining. After the primary antibody amplifier (linker) binds to the primary antibody, the polymer further binds to the linker/primary antibody complex, amplifying the signal. The HRP enzyme catalyzes the oxidation of 3,3'-diaminobenzidine (DAB) to form a brown precipitate at the antigen site.
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Research Field
Immunology
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Product Categories
Reagent
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Host
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Reactivity
Mouse,Rabbit
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Application
IHC-F,IHC-P
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Dilution Ratio
Ready-to-use
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Molecular Weight
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Clonality
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Clonality No.
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Isotype
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Immunogen
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Purification
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Conjugation
HRP
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Modification
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Form
liquid
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Buffer System
Reagent 1:Primary Antibody Amplifier;Reagent 2:HRP-Labeled Secondary Antibody Polymer
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Storage
Store at 2-8°C. Avoid freezing.
应用缩写
WB:蛋白质印迹法IHC-P:石蜡切片样本的免疫组织化学法IHC-F:冰冻切片样本的免疫组织化学法ICC/IF:免疫细胞荧光 IP:免疫沉淀法FC:流式细胞术ChIP:染色质免疫沉淀法ELISA:酶联免疫吸附测定
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