货号: 18006
别名: -
应用: IHC-P
反应种属: Mouse,Rabbit
目录价格:
RMB(含税报价)
数量: - + (库存件)
查看代理商 预计货期:3-4个工作日 说明书PDF
产品详情
  • Product Name
    Goat Anti-Mouse/Rabbit Multiplex IHC Detection Kit (Quintuple)
  • Gene ID
    -
  • SwissProt ID
    -
  • Gene Name
    -
  • Alternative Names
    -
  • Background
    Tyramide Signal Amplification (TSA) is a kind of enzymatic detection method that uses horseradish peroxidase (HRP) to label target proteins. Similar to the DAB chromogenic method of conventional immunohistochemistry, TSA also uses HRP-labeled secondary antibody that HRP catalyzes the tyramide fluorescein substrates added to the reaction system to generate activated fluorescent substrates, which can be covalently bound to residues such as tyrosine on the antigen. Therefore, the sample can be stably bound to tyramide fluorescein. The covalently attached protein cannot be washed off, even if the slides are treated to remove the antibodies, since the tyramide bond is covalent. The non-covalently bound antibody-HRP complex can be washed away by heat-induced epitope retrieval, and new antibody-HRP complex is repeated in the next round of incubation with another tyramide fluorescein substrate, thus achieving multiple-labeling. Protocol: 1. After antigen retrieval to secondary antibody incubation, the TSA Dye (added TSA Enhancer) should be incubated about 10-15 min at RT in dark. Wash the slides 3 x 5 min in PBS. 2. Repeat steps from antigen retrieval to TSA Dye reaction until all kinds of tyramide fluorescein substrates are incubated. Wash the slides 3 x 5 min in PBS. 3. Mount using compatible mounting medium and add a coverslip.
  • Research Field
    Immunology
  • Product Categories
    Reagent
  • Host
    Goat
  • Reactivity
    Mouse,Rabbit
  • Application
    IHC-P
  • Dilution Ratio
    TSA Enhancer : TSA Dye = 1 : 500
  • Molecular Weight
    -
  • Clonality
    Polyclonal Antibody
  • Clonality No.
    -
  • Isotype
    -
  • Immunogen
    -
  • Purification
    -
  • Conjugation
    HRP
  • Modification
    -
  • Form
    Liquid
  • Concentration
    -
  • Buffer System
    Reagent 1: Goat anti-Mouse/Rabbit HRP polymer Reagent 2: TSA Enhancer Reagent 3: TSA-520 Dye (Ex: 488nm / Em: 520nm) Reagent 4: TSA-570 Dye (Ex: 555nm / Em: 570nm) Reagent 5: TSA-620 Dye (Ex: 590nm / Em: 620nm) Reagent 6: TSA-690 Dye (Ex: 630nm / Em: 690nm) Reagent 7: TSA-780 Dye (Ex: 750nm / Em: 780nm)
  • Storage
    Refer to the instructions
验证详情

应用缩写

WB:蛋白质印迹法IHC-P:石蜡切片样本的免疫组织化学法IHC-F:冰冻切片样本的免疫组织化学法ICC/IF:免疫细胞荧光 IP:免疫沉淀法FC:流式细胞术ChIP:染色质免疫沉淀法ELISA:酶联免疫吸附测定 正能生物产品售后保障范围仅限官网产品详情页中“应用”一栏写明的实验应用,请参照上方的缩写含义,仔细对照后选择合适的产品。
用户反馈
    推荐排序
  • 最近评价
  • 推荐排序
产品评论
评论内容:
商品评分
微信扫码关注
Copyright ©2022 - 2023 成都正能生物技术有限责任公司
犀牛云提供企业云服务